Role for the fission yeast RecQ helicase in DNA repair in G2.

Members of the RecQ helicase subfamily are mutated in several human genomic instability syndromes, such as Bloom, Werner, and Rothmund-Thomson syndromes. We show that Rqh1, the single Schizosaccharomyces pombe homologue, is a 3'-to-5' helicase and exists with Top3 in a high-molecular-weight complex. top3 deletion is inviable, and this is suppressed by concomitant loss of rqh1 helicase activity or loss of recombination functions. This is consistent with RecQ helicases in other systems. By using epistasis analysis of the UV radiation sensitivity and by analyzing the kinetics of Rhp51 (Rad51 homologue), Rqh1, and Top3 focus formation in response to UV in synchronized cells, we identify the first evidence of a function for Rqh1 and Top3 in the repair of UV-induced DNA damage in G(2). Our data provide evidence that Rqh1 functions after Rad51 focus formation during DNA repair. We also identify a function for Rqh1 upstream of recombination in an Rhp18-dependent (Rad18 homologue) pathway. The model that these data allow us to propose helps to reconcile different interpretations of RecQ family helicase function that have arisen between work based on the S. pombe system and models based on studies of Saccharomyces cerevisiae SGS1 suggesting that RecQ helicases act before Rad51

Preference-for-Solitude and Adjustment Difficulties in Early and Late Adolescence

Social withdrawal has been associated with adjustment difficulties across development. Although much is known about shyness, little is known about preference-for-solitude; even less is known about its relations with adjustment across different periods of adolescence. We examined whether preference-for-solitude might be differentially associated with adjustment difficulties in early and late adolescence. Self- and parent-reports of withdrawal motivations and adjustment were collected from 234 eighth graders (113 boys; M age = 13.43) and 204 twelfth graders (91 boys; M age = 17.25). Results from structural equation modeling demonstrated that above and beyond the effects of shyness, preference-for-solitude was more strongly associated with adjustment difficulties in 8th grade than in 12th grade. Preference-for-solitude was associated with greater anxiety/depression, emotion dysregulation, and lower self-esteem in 8th grade; these relations were not found in 12th grade. Although preference-for-solitude was associated with lower social competence in both 8th and 12th grades, this relation was significantly stronger in 8th grade than in 12th grade. Findings suggest preference-for-solitude has closer ties to maladjustment in early adolescence than in late adolescence. Interventions targeting preferred-solitary youth in early adolescence may be particularly fruitfu

Deep rest-frame far-UV spectroscopy of the giant Lyman-alpha emitter 'Himiko'

We present deep 10h VLT/XSHOOTER spectroscopy for an extraordinarily luminous and extended Lya emitter at z=6.595 referred to as Himiko and first discussed by Ouchi et al. (2009), with the purpose of constraining the mechanisms powering its strong emission. Complementary to the spectrum, we discuss NIR imaging data from the CANDELS survey. We find neither for HeII nor any metal line a significant excess, with 3 sigma upper limits of 6.8, 3.1, and 5.8x10^{-18} erg/s/cm^2 for CIV $\lambda$1549, HeII $\lambda$1640, CIII] $\lambda$1909, respectively, assuming apertures with 200 km/s widths and offset by -250 km/s w.r.t to the peak Lya redshift. These limits provide strong evidence that an AGN is not a major contribution to Himiko's Lya flux. Strong conclusions about the presence of PopIII star-formation or gravitational cooling radiation are not possible based on the obtained HeII upper limit. Our Lya spectrum confirms both spatial extent and flux (8.8+/-0.5x10^{-17} erg/s/cm^2) of previous measurements. In addition, we can unambiguously exclude any remaining chance of it being a lower redshift interloper by significantly detecting a continuum redwards of Lya, while being undetected bluewards

Computer Model for Dynamic Skyline Behaviour

The development and experimental verification of a numerical model for the dynamic behavior of a cable logging system skyline is discussed. The model is intended to simulate the skyline behavior after a turn of logs breaks out of a "hang-up" on the ground. Output from the model may be used as a forcing function for a dynamic load on the tailspar or other component of the cable logging system. The numerical model uses finite difference and Runge-Kutta techniques. Output from the model consists of time-histories of the fluctuations in skyline tensions. From this output the frequencies of the skyline vibrations may be determined. The model was verified by experimental data collected while operating a small cable logging system in Oregon State University's McDonald Research Forest

Dynamic Characteristics of a Small Skyline Logging System with a Guyed Tailspar

A series of dynamic loading tests were conducted on a small skyline logging system (15.8 mm [5/8 inch] skyline) operating in a second-growth Douglas fir stand. The tests included free vibration tests and logging tests with turns weighing from 1.5 to 9 kN [340 to 2050 lbs]. Natural frequency and damping were evaluated from free vibration tests, and the free vibration portion of logging tests. Dynamic load magnitude was evaluated for logging tests with natural and artificial breakouts of turns with a range in turn weights, and for a series of logging tests with the same turn. The natural frequencies of the guylines were in good agreement with simple cable theory. However, the presence of the carriage on the skyline resulted in measured natural frequencies significantly lower than simple cable theory would predict. Damping of the tailspar system and the skyline averaged about 10% of critical damping, but was highly variable from test to test. Dynamic load magnitude, whether expressed as the load peak produced by turn break-out, or the maximum cyclic load, was highly variable, with coefficients of variation ranging from 31 to 79%. Even a series of logging tests with the same two-log turn produced maximum cyclic loads with a coefficient of variation of nearly 40%

Contribution of Scalar Loops to the Three-Photon Decay of the Z

I corrected 3 mistakes from the first version: that were an omitted Feynman integration in the function f^3_{ij}, a factor of 2 in front of log f^3_{ij} in eq.2 and an overall factor of 2 in Fig.1 c). The final result is changed drastically. Doing an expansion in the Higgs mass I show that the matrix element is identically 0 in the order (MZ/MH)^2, which is due to gauge invariance. Left with an amplitude of the order (MZ/MH)^4 the final result is that the scalar contribution to this decay rate is several orders of magnitude smaller than those of the W boson and fermions.Comment: 6 pages, plain Tex, 1 figure available under request via fax or mail, OCIP/C-93-5, UQAM-PHE-93/0

Initial results from the Caltech/DRSI balloon-borne isotope experiment

The Caltech/DSRI balloonborne High Energy Isotope Spectrometer Telescope (HEIST) was flown successfully from Palestine, Texas on 14 May, 1984. The experiment was designed to measure cosmic ray isotopic abundances from neon through iron, with incident particle energies from approx. 1.5 to 2.2 GeV/nucleon depending on the element. During approximately 38 hours at float altitude, 100,000 events were recorded with Z or = 6 and incident energies approx. 1.5 GeV/nucleon. We present results from the ongoing data analysis associated with both the preflight Bevalac calibration and the flight data